帕特里夏·J. Hilleren

Associate Professor of 医学杂志ogy 和 chair

地址:
生物系- CIS 210A
火博体育大学
萨拉托加泉，纽约州12866

电话:
(518) 580-8301

传真:
(518) 580-5071

电子邮件:
phillere@zizhanggui.com

教育:

• B.S. 微生物学(1987. 云州立大学，圣. 云、锰
• Ph.D. Biochemistry, 摩尔ecular 医学杂志ogy, 和 Biophysics (1997) University of Minnesota, 明尼阿波利斯、锰
• Postdoctoral Research (1997-2003) Howard Hughes Medical Institute, University of Arizona, 亚利桑那州图森市

课程:

• 通识科学I (LS I)
• 分子细胞生物学(BI 242)
• Chromatin Structure, Maintenance 和 Function (BI 360)
• mRNA Synthesis, Processing, 和 Turnover (BI 363)

主要研究方向:

All cells share a set of fundamental processes (DNA replication, RNA 和 protein synthesis 和 cell division) that are each subject to quality control. 这确保了错误 are recognized 和 dealt with before they can become a problem for the cell. 质量 control is also important in the pathway of gene expression. 在这条道路上，新 transcribed pre-mRNA undergoes a number of processing steps that together yield a 成熟的信使核糖核酸. These processing steps happen in the nucleus, in some cases, while the newly made pre-mRNA is still in very close proximity to its corresponding gene. 一次 processing is complete, the mRNA is exported from the nucleus into the cytoplasm of the cell where it can function in protein synthesis. Nuclear pre-mRNA processing steps are very fast 和 as a consequence of speed, mistakes can happen that yield defective mrna. If defective mrna are allowed to persist 和 accumulate in the cell, they could lead to the production of mutant or deleterious forms of proteins.

Fortunately, quality control systems are in place to recognize 和 degrade defective mrna. The best-characterized mRNA quality control systems operate in a translation dependent manner that works in the cytoplasm during protein synthesis. 例如, mrna that carry early premature stop codons are detected 和 rapidly degraded by a pathway termed nonsense-mediated decay. Alternatively, mrna that are defective because they do not possess a stop codon are also recognized as aberrant 和 are rapidly degraded, in this case by the non-stop mediated decay pathway.

In contrast to the well-characterized cytoplasmic systems, quality control systems that operate during pre-mRNA processing in the nucleus are poorly understood, at best. Depending upon the nature of the defect, aberrant pre-mrna accumulate within the nucleus, in some cases at or near their sites of transcription. 为什么这些异常 RNAs accumulate near the gene, 和 how they are eventually removed remain puzzling 问题.

Using the budding yeast, Saccharomyces cerevisiae, my research is aimed at underst和ing the mechanisms that underlie nuclear quality control systems that operate to ensure 基因表达的准确性. Current 和 future research projects address the following 问题:

1. how aberrant RNAs are differentiated from normal mrna by the various degradation 系统;

2. the mechanism by which some aberrant RNAs are retained at the site of transcription; 和

3. the consequence to continued gene expression (if any) from the nuclear accumulation 异常RNA.

出版物:

Hilleren,帕特丽夏 和罗伊·帕克. (2003) Cytoplasmic Degradation of Splice Defective pre-mRNA 和 Intermediates: A Dbr1p-Dependent 质量 Control System for pre-mRNA Splicing. 分子细胞. 12:1453-65.

Hilleren 等., (2001) 质量 Control of mRNA 3-end Processing is Linked to the Nuclear 外来体. 自然. 413:438-42.

Hilleren,帕特丽夏 和罗伊·帕克. (2001) Defects in the mRNA Export Factors Rat7p, Gle1p, Mex67p 和 Rat8p Cause Hyperadenylation During 3'-end Formation of Nascent Transcripts. RNA 7:756 - 64.

Hilleren,帕特丽夏 和罗伊·帕克. (1999) mRNA Surveillance in Eukaryotes: Kinetic Proofreading of Proper Translation Termination as Assessed by mRNP Domain Organization? RNA 5:711 - 719

Hilleren,帕特丽夏 和罗伊·帕克. (1999) Mechanisms of mRNA Surveillance in Eukaryotes. 安. 牧师. 遗传学. 33:229-60.

帕特里夏·J·海伦.，高，红英，和Paul G. Siliciano. (1995) The Amino-terminal Domain of Yeast U1-70K is Necessary 和 Sufficient for Function. 摩尔. 细胞. 医学杂志. 15:6341-50.

克莱尔·C·波默罗伊., 帕特里夏·J·海伦.， M. 科林·乔丹. (1991) Latent murine Cytomegalovirus DNA in Splenic Stromal 小鼠细胞. 日记账. 病毒学 65:-3330-04.